A Preliminary Study on Diversity of Endophytic Fungi from Wild Dendrobium officinale under Different Habitats

The nutrition roots of D.officinale in growth period collected from two different habitats:Lang mountain natural heritage scenic area of Xinning county,Hunan province and Orchid resources gene garden of Yachang orchid nature reserve of Leye county,Guangxi province as site of collection of wild D.officinale.The endophytic fungi was isolated in tissue blocks cultivation,and identified by using morphological and molecular techniques.In this study,34 fungi species were isolated from the 41 representative fungi strains.The diversity of endophytic fungi with the phylogenetic diversity of fungi isolate were investigated.The phylogenetic tree indicated that the fungi mainly belonged to Xylariales and Hypocreales of Ascomycota,and Tulasnellaceae of Basidiomycota.The Ascomycota was dominant,containing 31 species.The analysis results of Simpson diversity index indicated that the diversity of endophytic fungi from D.officinale exhibited high diversity,the diversity of Yachang were higher than of Lang mountain.