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Mutation of cellulase-producing Trichoderma viride strain

Hongyan Zhang

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Abstract

The cellulose-producing Trichoderma viride stain was mutated with diethyl sulfate(DES) and microwave.The results showed that the filter paper activity(FPA) of the strain DES8,which was mutated by DES,was 27.67% higher than that of original strain.And the FPA of the strain WB8,which was microwave mutated strain of DES8,was 14.88% higher than that of DES8.Compared with the original strain,FPA of strain WB8 was improved by 46.67% and the CMCase activity of WB8 was improved by 42.37%.Further study indicated that the strain WB8 was genetic stable.

About this research paper

What this paper is about

The cellulose-producing Trichoderma viride stain was mutated with diethyl sulfate(DES) and microwave.The results showed that the filter paper activity(FPA) of the strain DES8,which was mutated by DES,was 27.67% higher than that of original strain.And the FPA of the strain WB8,which was microwave mutated strain of DES8,was 14.88% higher than that of DES8.Compared with the original strain,FPA of strain WB8 was improved by 46.67% and the CMCase activity of WB8 was improved by 42.37%.Further study indicated that the strain WB8 was genetic stable.

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Available abstract

The cellulose-producing Trichoderma viride stain was mutated with diethyl sulfate(DES) and microwave.The results showed that the filter paper activity(FPA) of the strain DES8,which was mutated by DES,was 27.67% higher than that of original strain.And the FPA of the strain WB8,which was microwave mutated strain of DES8,was 14.88% higher than that of DES8.Compared with the original strain,FPA of strain WB8 was improved by 46.67% and the CMCase activity of WB8 was improved by 42.37%.Further study indicated that the strain WB8 was genetic stable.

Key concepts: Strain (injury), Cellulase, Trichoderma viride, Mutation breeding, Cellulose, Filter paper, Chemistry, Food science

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