Expressions of MMP-2,MMP-9 and its Tissue Inhibitor TIMP-1,TIMP-2 in Esophageal Carcinoma and its Clinical Significance

Objective To investigate the expressions of matrix metalloproteinase-2,-9(MMP-2,MMP-9) and tissue inhibitor of metalloproteinase-1,-2(TIMP-1,TIMP-2) in esophageal carcinoma tissue and its correlation with invasion and metastasis.Methods MMP-2,MMP-9,TIMP-1,TIMP-2 proteins were detected by immunohistochemistry technique in 45 cases of esophageal squamous cell carcinoma(SCC).Results The positive rates of MMP-2、MMP-9、TIMP-1、TIMP-2 protein expression were 80.0%、 17.8%、13.3% and 84.4%.MMP-2,MMP-9 protein were mainly expressed in cancer cell,and TIMP-1,TIMP-2 in mesenchyma cell.The expressions of MMP-2、MMP-9、TIMP-1、TIMP-2 protein were not significantly different in cancer with different differentiation degree.The positive rate of MMP-2 expression was significantly higher in deeply invasive tissue than in superficially invasive tissue(χ 2=4.375,P0.05),and it was also markedly higher in cases with lymph nodes metastases than in cases without(χ 2= 6.252,P0.01).Conclusion MMP-2,MMP-9 protein are mainly expressed in cancer cells,and TIMP-1,TIMP-2 are mainly expressed in mesenchyma cells.The expressions of MMP-2、MMP-9、TIMP-1、TIMP-2 are not correlated with the cancer cell differentiation.The expression of MMP-2 is related to invasion and lymph nodes metastases of the cancer.