Effect and relationship of KISS-1 and Matrixmetalloproteinase-9 on invasion and metastasis of gastric carcinoma

Purpose To investigate the effect and relationship of metastasis suppressor gene KISS-1 and matrixmetalloproteinase-9(MMP-9) on invasion and metastasis of gastric carcinoma.Methods The eukaryotic expression vector pcDNA3.1-KISS-1 was transfected into gastric carcinoma cell line BGC-823 by lipofectamine.To observe the expression of MMP-9(Western blotting and RT-PCR) and potential of invasion(Transwell in vitro) in transfected BGC-823.Results After pcDNA3.1-KISS-1 recombinant vector was successfully constructed in BGC-823 cells,the expressions of KISS-1 protein and mRNA in cells transfected with pcDNA3.1-KISS-1 were significantly higher than it in cells transfected with pcDNA3.1 and control cells(P0.05).The expression of MMP-9 protein and mRNA in cells transfected with pcDNA3.1-KISS-1 were significantly lower than it in cells transfected with pcDNA3.1 and control cells(P0.05).Cell number invading through Matrigel filter in cells transfected with pcDNA3.1-KISS-1 was significantly decreased compared with that in cells transfected with pcDNA3.1 and control group(P0.05).The inhibition rate of invading ability reached 25%.Conclusion The effective transfection of pcDNA3.1-KISS-1 can decrease the expression of MMP-9 and inhibit the potential of invasion of BGC-823 cells.The depressant effect of KISS-1 gene on invasion and metastasis of gastric carcinoma may implement by decreasing the action of MMP-9.