Effects of Transforming Growth Factor β_1 on the Osteoclast-like Cells in vitro

Objective:To study the effects of transforming growth factor β 1 on the osteoclast like cell in vitro.Methods:The acid phosphatase(ACP)and tartrate resistant acid phosphatase(TRAP)activities were measured kineticly and the introcellular hydrogen ions were calculated on the confocal laser scanning microscope.The area and number of the resorption pits were determined with the Leica Quantimet 500 system Scanning.Electronicmicroscopy shows the area of bone resorption pits.Results:The ACP and TRAP activity had difference( P 0.01)when the density of transforming growth factor β 1 was 10 -2 ng/ml,10 -1 ng/ml,1ng/ml and 5ng/ml.The area and number of the resorption pits were significant( P 0.01)when the density of transforming growth factor β 1 was 10 -2 ng/ml,10 -1 ng/ml,1ng/ml and 5ng/ml.The hydrogen ions release could not significantly be inhabitted by transforming growth factor β 1.Conclusions:The transforming growth factor β 1 could not inhabit hydrogen ions,but could decrease osteoclastic bone resorption due to the TRAP.