2003•Tianjin Yike Daxue xuebaoRequires access
The expression of Bcl-2and Bax after focal cerebral ischemia-reperfusion in rat
Wang Jizuo
Open publisher page 0 citations
Abstract
Objective:To study the change of Bcl-2and Bax expression following focal cerebral ischemia-reperfusion in rat.Methods:The focal cerebral ischemia-reperfusion model was made by thread embolisk of middle cerebral artery(MCA),using immunohistochemical technique in analyzing the expression of Bcl-2and Bax.Results:During reperfusion after2h of ischemia,the expression of Bcl-2was induced in the ischemic cortex with peak expression at6h,and then decreased.The expression of Bax was observed markedly in the ischemic cortex at early stage of reperfusion.Immunoreactivity of Bax increased with time after reperfusion with peak expression at24～48h.The ratio of Bcl-2/Bax increased at the beginning of reperfusion with peak at6h,decreasing in the following time.Conclusion:The change of Bcl-2/Bax ratio would play a vital role in regulating the neuronal survival or death during reperfusion after focal cerebral ischemia.