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OPTIMIZATION OF EXTRACTION PROCESS OF CHLOROGENIC ACID IN FLOS LONICERAE BY ORTHOGONAL TEST

Tiehua Zhao

Open publisher page 1 citations

Abstract

Objective:To optimize the water extraction process of chlorogenic acid in Flos lonicerae.Methods:The extraction process of chlorogenic acid in Flos lonicerae was optimized by orthogonal test,the extracting rate of chlorogenic acid as index and ultraviolet spectrophotometry as detection method.Results:The optimum extraction condition was:the particle size of medicinal substances was 60 screen meshes,15 fold water(pH=4)marinated 3 times at 70℃,and 1h each time.Conclusions:This method is easy to operate,has better reproducible and high extracting rate of chlorogenic acid.

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What this paper is about

Objective:To optimize the water extraction process of chlorogenic acid in Flos lonicerae.Methods:The extraction process of chlorogenic acid in Flos lonicerae was optimized by orthogonal test,the extracting rate of chlorogenic acid as index and ultraviolet spectrophotometry as detection method.Results:The optimum extraction condition was:the particle size of medicinal substances was 60 screen meshes,15 fold water(pH=4)marinated 3 times at 70℃,and 1h each time.Conclusions:This method is easy to operate,has better reproducible and high extracting rate of chlorogenic acid.

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Available abstract

Objective:To optimize the water extraction process of chlorogenic acid in Flos lonicerae.Methods:The extraction process of chlorogenic acid in Flos lonicerae was optimized by orthogonal test,the extracting rate of chlorogenic acid as index and ultraviolet spectrophotometry as detection method.Results:The optimum extraction condition was:the particle size of medicinal substances was 60 screen meshes,15 fold water(pH=4)marinated 3 times at 70℃,and 1h each time.Conclusions:This method is easy to operate,has better reproducible and high extracting rate of chlorogenic acid.

Key concepts: Chlorogenic acid, Flos, Extraction (chemistry), Chromatography, Chemistry, Spectrophotometry, Biochemistry, Antioxidant

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