Tetrandrine inhibited IκB-α phosphorylation regulated the expression of TNF-α、IL-6 during rat myocardial ischemic/reperfusion

Objective:To investigate the effect and mechanism of tetrandrine(Tet) on the phosphorylation of IκB-α(P-IκB-α) and the expression of TNF-α,IL-6 during rat myocardial ischemic/reperfusion(IR) period.Methods:A total of 60 male Sprague-Dawley rats were randomly divided into 3 groups:Sham group,I/R group,Tet group.The Sprague-Dawley rat underwent 30 min of left anterior descending (LAD) coronary occlusion and 24 h reperfusion to make ischemia/reperfusion (I/R) injury model in vivo.Sham group were not subjected to occlusion of artery.Tet group were injected with tetrandrine to abdominal cavity 20 min before ischemic starting.The other procedures were same to the I/R group.Samples were collected after 24h reperfusion.The expression level of TNF-α and IL-6 protein was detected by ELISA.The expression of P-IκB-α was observed by immunohistochemical stain and image analysis.Results:In Tet group the expression of TNF-α 、IL-6 were significant lower compared with those of I/R group[(208.40±25.12)pg/ml vs.(306.65±17.78)pg/ml,(587.40±137.40)pg/ml vs.(1003.75±138.33)pg/ml,P0.01 all] and significant higher than shame group respectively[(208.40±25.12)pg/ml vs.(61.45±9.20)pg/ml,(587.40±137.40)pg/ml vs.(229.25±90.13)pg/ml,P0.01 all).In Tet group the expression of P-IκB-α were significant lower than that of I/R group[(0.0817±0.09)pg/ml vs.(0.1755±0.01)pg/ml,and significant higher than that of shame group[(0.0817±0.09)pg/ml vs.(0.0513±0.03),P0.01 all].Conclusion:Tetrandrine can attenuate myocardial ischemia/reperfusion injury,inhibit the IκB-α phosphorylation and reduce the harmful cytokine TNF-α and IL-6.