2005•Journal of Zhejiang University(Sciences Edition)Requires access
Analysis of the genetic diversity of Hepatacodium miconioides at different altitude in Tiantai mountain in Zhejiang Province and its relationship with environmental factors
Junmin Li
Open publisher page 2 citations
Abstract
The genetic diversity within populations and genetic differentiation among populations of Hepatacodium miconioides at three different altitudes in Tiantai mountain in Zhejiang Province were analyzed by random amplified polymorphic DNA (RAPD) technique. The relationships between the genetic diversity and the environmental factors were analyzed. 12 random primers were selected in the amplification of 60 plants and 122 repetitive loci were produced. The percentage of polymorphic loci of three populations ranged from 18.85% to 23.77% with an average of 21.86% indicating the relatively low genetic diversity of H. miconioides. The averages of Shannon index of phenotypic diversity (0.132 9) and Nei index (0.092 5) within populations were relatively low. The distinct genetic differentiation existed among populations of \%H. miconioides\% in spite of the relatively small geographical distribution. Estimated by Shannon index of phenotypic diversity, the average genetic diversity within populations of H. miconioides accounted for 33.58% of the total genetic diversity while the genetic diversity among populations accounted for 66.42%. The genetic differentiation among populations of H. miconioides, as estimated by Nei index, was 0.6546. The gene flow estimated from Gst was only 0.265 6, indicating that the gene flow among populations of H. miconioides was relatively low. The average of the genetic identity among populations of H. miconioides was 0.712 6 and the average of genetic distance of H. miconioides was 0.341 2. The genetic identity between population at the altitude of 990 m and populations at the altitude of 780 m was the highest. The genetic identity between populations at the altitude 500 m and other two populations was relatively low. The correlation analysis showed that the genetic diversity within populations was significantly positively related with the soil total nitrogen.