The discuss of protective action of safflower injection post-treatment on ischemical reperfusion injury myocardium in rat

Objective To observe the effect of safflower injection post-treatment on myocardial ischemial reperfusion(I/R) injury in the matrix metalloproteinase-2(MMP-2),tissue inhibitor of metalloproteinase-2(TIMP-2) of the mRNA expression level and MMP-2/TIMP-2 mRNA ratio of the initial treatment of safflower injection post-treatment on the possibility of I/R injury and the possible mechanism of myocardial.Methods Established myocardium I/R injury model,ischemia(I) 30min and reperfusion(R) 30min as the observation point.50 healthy SD rats were randomly divided into 5 groups:sham-operation group(S group),ischemia group(I group),I/R group,low dose safflower injection group(L group),high dose safflower injection group(H group),each of 10.Analyzed the levels of MMP-2 and TIMP-2 mRNA in myocardium by RT-PCR.ResultsThe level of MMP-2 mRNA of I group and I/R group were significantly higher than that of S group(P0.05),while expression level of TIMP-2 mRNA was significantly lower than that of S group(P0.05),MMP-2/TIMP-2 mRNA were significantly higher than that of S group(P0.05);And the expression level of MMP-2 mRNA of I/R group was higher than that of I group(P0.05),the expression level of TIMP-2 mRNA was lower than that of I group(P0.05),MMP-2/TIMP-2 ratio was higher than that of I group(P0.05).L group and H group compared with I/R group,the expression level of MMP-2 mRNA decreased significantly(P0.05),while TIMP-2 mRNA expression was significantly increased(P0.05),MMP-2/TIMP-2 mRNA ratio was significantly lower(P0.05).Conclusion Safflower injection post-processing can improve the I/R myocardial injury,and by adjusting the ratio of MMP-2/TIMP-2 to protect I/R injury in cardiac tissue.