Analysis of Components in Aqueous of Paeonia Lactiflora Pall and Paeonia Lactiflora Pall Preparation with Wine by Solid Phase Extraction-High Performance Liquid Chromatography-Electrospray Ionization Mass Spectrometry

A solid phase extraction and high performance liquid chromatography-electrospray ionization-mass spectrometry was developed for the analysis and identification of components in the aqueous of Paeonia lactiflora Pall and Paeonia lactiflora Pall preparation with wine.The ultraviolet chromatogram,total ion current chromatogram and extracted ion chromatogram of the samples and ESI/MS/MS spectra of the peaks in the chromatograms were obtained in order to identify the structure of compounds.It showed that there was a same chemical composition in the aqueous of P.lactiflora Pall and P.lactiflora Pall preparation with wine.But phenolic compounds in the aqueous of P.lactiflora Pall preparation with wine suffered some degree of lowered of the content.The sixteen identified compounds were sucrose,(+)-catechin,mudanpioside E,lactiflorin,albiflorin,oxpaeoniflorin,paeoniflorin,oxypaeoniflorin,galloylpaeoniflorin,mudanpiosideⅠ,benzoylpaeoniflorin,trigalloyglucose,tetragalloyglucose,pentgalloyglucose.Based on the above analysis,one role of preparation with wine was to alleviate the side effects on the liver and to reserve the original effects of P.lactiflora Pall at the same time.