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Study on Apoptosis of Cerebral Cells Following HIE by Using Flow Cytometry

Kai Liu

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Abstract

Objective:To determine whether there is apoptosis in cerebral cortical cells of the neonate pigs with hypoxia-ischemia(HI)injury.Methods:We have abserved the DNA Ladderby gel electrophoresis and the apoptosis rate of cerebral cortical cells via flow cytometry.Results:The results showed the apoptosis ratio of cerebral cortical cells in left frontotemporal corticocerebrum 72hr post asphyxia was higher than control group(n=15,12;means%)=26181635,323293;1).Conclusion:It suggest that apoptosis of cerbral cortical cells plays an important role in the delayed cerebral damage of the neonates post HI.

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Objective:To determine whether there is apoptosis in cerebral cortical cells of the neonate pigs with hypoxia-ischemia(HI)injury.Methods:We have abserved the DNA Ladderby gel electrophoresis and the apoptosis rate of cerebral cortical cells via flow cytometry.Results:The results showed the apoptosis ratio of cerebral cortical cells in left frontotemporal corticocerebrum 72hr post asphyxia was higher than control group(n=15,12;means%)=26181635,323293;1).Conclusion:It suggest that apoptosis of cerbral cortical cells plays an important role in the delayed cerebral damage of the neonates post HI.

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Available abstract

Objective:To determine whether there is apoptosis in cerebral cortical cells of the neonate pigs with hypoxia-ischemia(HI)injury.Methods:We have abserved the DNA Ladderby gel electrophoresis and the apoptosis rate of cerebral cortical cells via flow cytometry.Results:The results showed the apoptosis ratio of cerebral cortical cells in left frontotemporal corticocerebrum 72hr post asphyxia was higher than control group(n=15,12;means%)=26181635,323293;1).Conclusion:It suggest that apoptosis of cerbral cortical cells plays an important role in the delayed cerebral damage of the neonates post HI.

Key concepts: Apoptosis, Medicine, Flow cytometry, Cerebral blood flow, Cytometry, Cerebral cortex, Hypoxia (environmental), Pathology

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