Effects of CKZ on Mouse CD4~+ CD25~+ T Cells in vitro

To investigate whether Chinese medicine Chuankezhi(CKZ) functions in upregulation of CD4+ CD25+ regulatory T cells(Tr),fluorescence conjugated antibody labeling and flow cytometry were used to evaluate the effects of CKZ on expression of CD69 and CD25 on T cells induced by Concanavalin A(ConA).It was demonstrated that CKZ inhibited the expression of CD69,the early activation marker,and increased the expression of CD25 on T cells in response to ConA.These results suggested that CKZ had an inhibitory effect on T cell activation,and the increase in CD25 expression was not due to activation of T cells but a marker of upregulation of CD4+ CD25+ Tr.