2012•Guangdong nongye kexueOpen access

Purification and activity identification of antimicrobial peptides from bovine white blood cell

Hu JianHe

Abstract

Antimicrobial peptides were isolated and purified from bovine White blood cell,and their antimicrobial activity in vitro was analyzed.Antimicrobial peptides from the White blood cell of Chinese Holstein were purified by ion exchange chromatography and purified by RP-HPLC.Antimicrobial activity of purified peptide was tested by the agarose radial diffusion assay.The result showed that the positive ion peak acquired by ion exchange chromatography had antimicrobial activity.Six peaks acquired by RP-HPLC had the activity against E coli and Salmonella,peak F1,peak F2 and peak F3 had the activity against Staphylococcus aureus,only peak F1 had the activity against Candida albicans.

About this research paper

What this paper is about

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Antimicrobial, Candida albicans, Antimicrobial peptides, Agarose, High-performance liquid chromatography, Peptide, Chemistry, Ion chromatography