2009•Di-Si Junyi Daxue xuebaoRequires access
Protective effects of estradiol on angiopoietin-1 mRNA expression and bcl-2 gene expression in castrated rats with focal cerebral ischemia after reperfusion
Cheng Xiao
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Abstract
AIM:To investigate the protective effect of estradiol on angiopoietin-1 mRNA(Ang-1 mRNA) and bcl-2 gene expression in castrated rats with focal cerebral ischemia reperfusion.METHODS:Cerebral ischemia reperfusion models were established in female Wistar rats and the rats were divided into WT castrated rat placebo group(A group),estradiol benzoate group(B group),ERKO castrated rat placebo group(C group) and estradiol benzoate group(D group).The plasma estradiol levels,changes in cerebral blood flow,Ang-1 mRNA,apoptosis and bcl-2 gene expressions were determined respectively by radio-immunity analysis,laser Doppler flowmetry(LDF),immunohistochemical stain and reverse transcription polymerase chain reaction(RT-PCR).RESULTS:The plasma estradiol levels in WT and ERKO castrated rats treated with estradiol were higher than those in respective control groups(P0.01).In group of WT MCAO castrated rats treated with estradiol,the change in cerebral blood flow after cerebral ischemia reperfusion,the Ang-1 mRNA expression and bcl-2 gene expression in brain tissues were higher than those for their respective control(P0.01).CONCLUSION:The increased expression of Ang-1 mRNA and bcl-2 gene expression mediated by estradiol and their stabilizing effect on capillary vessels are the important repair mechanism of cerebral ischemia reperfusion injury.