Construction of Arabidopsis Gene bZIP23 Overexpression Vector and Screening of Expression Plant

[Objective]Arabidopsis were used as material to clone bZIP23 gene,we construct gene bZIP23 overexpression vector and screen expression plant.[Method]Total RNA was extracted from Arabidopsis seedlings,and cDNA fragments of bZIP23 gene were amplified by RT-PCR. Using the restriction enzymes and T4 DNA ligase,cDNA fragments were subsequently cloned into PART27 vectors,and then were transformed into Trans-T1 phage resistant chemically competent cells. Analysis of bacterial colony PCR and cDNA sequencing were performed to confirm that cDNA of the Arabidopsis thaliana bZIP23 gene was successfully cloned. The recombinant plasmids were obtained and transformed into Agrobacterium GV3101 cells. Wild-type Arabidopsis thaliana was transformed by using floral-dip method.[Result] Analysis of bacterial colony PCR and DNA sequencing were performed to confirm recombinant plasmids,complementary and overexpression positive plants were obtained through genetic screening and identification of genetically modified methods. [Conclusion] Construction of Arabidopsis gene bZIP23 overexpression vector and screening of expression plant laid the foundation for the function of gene bZIP23.