Construction and primary analysis of peanut pericarp full-length cDNA library

A full-length cDNA library from the pericarp of Minhua 6 peanut was constructed based on switching mechanism at 5′end of RNA transcript(SMART) system.The purified double strand cDNA was ligated to SfiⅠ digested vector pDNR-LIB,and transformed into DH5α by electroporation.The entry library constructed has a high titer of 1.3×107 cfu.PCR results showed that the inserts varied from 750 to 2000 bp with average size larger than 1000 bp and 95.5% of recombinant percentage.As results bioinformatics analysis,about 68% of sequences were full-length.Thirteen sequences with known functions were identified by BlastX searched against the NCBI non-redundant protein databases.These results will be useful for screening and cloning pericarp special expression genes and adding genetic information for peanut.