CULTURE OPTIMIZATION OF PRODUCED Mn-PEROXIDASE(MnP) BY PHANEROCHAETE CHRYSOSPORIUM 5.776

White-rot fungi Phanerochaete chrysosporium 5. 776 produce extracellular ferment MnP. In high-Mn low-nitrogen medium,high enzyme activity of Mn-peroxidase (MnP) was detected. Under the optimum condition study of initially fermentation culture,optimizing initially culture (gram per liter): glucose, 10;diammonium tartrate,0.37(2mmol);Tween80,1; Mn~(2+), 9.9 μg; The culture temperature was 34℃.Under above conditions,the strain produced MnP activity up to 1 200 U·L~(-1) during 5 days stationary culture ,which increased almost 17 times than that of initially fermentation culture.