Hydrolysis Performance of Trichoderma viride EU2-77 Cellulase Against Horticultural Waste

In order to enhance the biomass hydrolysis efficiency,horticultural waste collected from Singapore was pretreated by organosolv extraction method.The pretreated and non-pretreated horticultural waste were hydrolyzed using the cellulolytic enzyme complex from Trichoderma viride EU2-77,respectively.After pretreatment,the reducing sugar yield was increased by 21.55 times due to the high β-glucosidase and xylanase activity in the enzyme samples.Biomass hydrolysis performances of the cellulolytic enzyme complex from the commercial strain T.reesei RUT C30,commercial cellulase Celic Ctec,Celluclast 1.5L,and the mixture of Celluclast 1.5L and Novozym 188 were compared.It was demonstrated that cellulolytic enzyme complex derived from strain T.viride EU2-77 yielded more reducing sugar(316.4 mg/g) than T.reesei RUT C30 cellulases(232.4 mg/g) and Celluclast 1.5L(239.6 mg/g).However,its performance was close to that of Celic Ctec(303.2 mg/g) and the mixture of Celluclast 1.5L and Novozym 188(331.6 mg/g),rendering the need for β-glucosidase supplementation in biomass hydrolysis unnecessary.With such promising enzymatic hydrolysis performance,cellulases derived from T.viride EU2-77 are much competitive in the bioconversion of lignocellulose to sugars.