EVALUATION OF THE EFFICACY OF SIX DRUGS AGAINST ACUTE MURINE TOXOPLASMOSIS

To find effective anti-toxoplasmosis drugs,commonly used six drugs were selected to evaluate their efficacy against acute murine toxoplasmosis.Kunming male mice were inoculated intraperitoneally with 1×104 tachyzoites of Changning(CN) strain of Toxoplasma gondii.Four hours later,these mice were given the following drugs by gavage or drinking water: Azithromycin(at the dose of 150,120,90 mg/(kg·d)),Tiamulin(at the dose of 100 mg/(kg·d)),Clarithromycin(at the dose of 15 mg/(kg·d)),Sulfachloropyrazine sodium(at the dose of 150,120,90 mg/(kg·d)),Metronidazole(at the dose of 150 mg/(kg·d)) and Trimethoprim(at the dose of 10 mg/(kg·d)).Mice were treated for 5-6 days.The experiment ended at 10 days after inoculation.Results showed that the average survival time of mice treated with Azithromycin or Sulfachloropyrazine sodium was significantly longer than that of non-treated group(P0.05),but the difference was not significant between the groups treated with Tiamulin,Clarithromycin,Metronidazole or Trimethoprim and non-treated group(P0.05).In comparison with non-treated group,the number of tachyzoites counted by microscopic examination of the peritoneal fluid reduced by 70% or more at 4-7 day in the group treated with azithromycin and reduced over 99% in group with Sulfachloropyrazine sodium.These results indicated that Sulfachloropyrazine sodium was the most effective drug against Toxoplasma gondii.The recommended dose and treatment period need to be addressed in further study.