Construction and Identification of Recombinant Adenoviral Plasmids Containing CXCL12 Gene

Objective: To construct and identify the recombinant adenoviral plasmids containing exogenous CXCL12 gene and EGFP gene and to investigate the expression of target genes.Methods: Genetic engineering techniques were used to construct the recombinant plasmids of pShuttle2-CXCL12-EGFP.The adenoviral plasmids pAdeno-CXCL12-EGFP were constructed by Adeno-X expression system.The plasmids were identified.Recombinant adenoviral vectors were packaged by cell line 293.The expression of EGFP was detected under the fluorescence microscopy.Results: The pShuttle2-CXCL12-EGFP was successfully constructed and identified by enzymolysis and sequencing.The adenoviral plasmids pAdeno-CXCL12-EGFP were successfully constructed and identified by enzymolysis and PCR.The expression of EGFP could be observed after the plasmids pAdeno-CXCL12-EGFP were transfected.Conclusion: The adenoviral plasmids containing CXCL12 gene and EGFP gene were constructed successfully by Adeno-X system.