Protein Composition Analysis ofCoix Lacryma-Jobi L.var.ma-yuen(Roman.) Stapf Seeds

Objective: To analyze the major proteins and amino acid composition of Coix seeds.Methods: Polarimetry,Soxhlet extraction and drying method were respctively used to analyze the starch,crude fat and water content.Muffle furnace was applied when the ash percentage was measured.Albumins,gulbulins,coixins and glutelins were sequentially extracted and then quantified.Amino acid content was measured by amino acid analyzer Hitachi L 8900.Results: The total protein content of Coix seeds was 14.17%,among which the contents of albumins,globulins,coixins and glutelins were 0.20,0.88,6.34 and 5.30 mg/100 mg fresh weight,accounting for 1.43%,6.20%,44.74% and 37.38% of total protein content,respectively.A total of 15 kinds of amino acid were detected in this research,including all essential and semi-essential amino acids for human beings except for Trp.There was a wide range of amino acid contents,from Glu,the highest composition(3.59 mg/100 mg) to Met,the lowest(0.17 mg/100 mg) one.Conclusions: Coixins and glutelins are the most abundant proteins ofCoix seeds,and this research provides valuable sights for exploring functional food from Coix seeds.