Carbendazim Resistance Strains from Trichoderma Harzianum Induced by UV-light

To realize beneficial interaction of biopesticide and chemical pesticide,the resistant Trichoderma harzianum strain T24-6 against Carbendazim was obtained by UV-light repeatedly inducing and Carbendazim stress on PDA medium.The inhibition rate of Carbendazim to T24-6 was 3.0% when Carbendazim concentration was 300 mg·L-1.Five treatments,Trichoderma,Carbendazim,three combinations of Trichoderma and Carbendazim with different weight rates 9:1,8:2 and 7:3 respectively,were designed in the experiment.In every treatment,the inhibition rate and the antagonism of vivo-strawberry were tested.The inhibition efficiency of Botrytis cinerea was 85% under the treatment with application of Trichoderma and Carbendazim at 8:2,higher than that in the treatment of applying Trichoderma T24-6(70.3%) and Carbendazim(69.6%) separately.Similarly,the efficiency of controlling strawberry mold disease caused by Botrytis cinerea was 83.9% by application of Trichoderma and Carbendazim at 8:2.The controlling effect was also better than the treatment of Trichoderma(67.9%) and Carbendazim(64.2%) separately.These results further demonstrated that the beneficial interaction of Trichoderma and Carbendazim was more advantageous.So,when using Trichoderma strain T24-6 to prevent and control crop diseases,adding a small amount of Carbendazim not only reduce the activity and ability of infection of plant pathogens but also improve the effects of biological control.