Preparation and Identification of Monoclonal Antibodies against Enrofloxacin

Preparation and identification of monoclonal antibodies (mAb) against Enrof loxacin (EFLX) Immunogen were carried out.EFLX-BSA was synthesized using the activated ester method,the hybridoma cell strain secreting anti-EFLX mAb was obtained by using hybridoma technology.The massive mAb was induced with in vivo method,the titer,affinity,sensitivity,specificity and subtype of the mAb were characterized.The hybridoma cell lines of 7C5 were screened for specificity to EFLX,the isotypes of the mAb were IgG1.The indirect ELISA titer of the mAb was 1.035 × 10-6in supernatant.The result had a lineardetection range from 2.05 ng/mL to 98.38 ng/mL (R2=0.992 7),with IC50and LOD values of 14.21 ng/mL and 0.56 ng/mL,the mAb had no cross reaction to other antibiotics.The antibody obtained in this experiment is of high utility and strong specificity,which may provide good antibody materials for the research anddevelopment of colloidal gold test and ELISA kit.