Screening and Identification for Breeding Genic Male Sterile Materials in Chinese Cabbage

Eight F1hybrids of Chinese cabbage were used as test materials,the fertility identification found that there were 5 materials expressing fertility separation and 100% fertile plants in the other 3materials.Selfing was applied to the 3 materials with 100% fertile plants.Among the F2system,there were 5 F2systems expressed sterile plants in 7 F2systems of Qinglvwang.The genetic method was designed based on Multiple Allele Hypothesis of Chinese Cabbageto identify male sterile alleleMsand restorer alleleMsfin 5 F2lines in which the fertility segregated ratio of fertile and sterile plants was 3∶1 and maintainer allelemsin 2 fertile F2lines.Manual pollination was applied to sibling,selfing and crossing and genetic male sterile ABline,temporary maintainer line and genic male sterile lines with 100% sterile plants were obtained,which will provide material foundation for further studying and utilizing the male sterile source.