Effect of Different Extenders on Tibetan Mastiff Semen Preservation

The Tibetan mastiff semen preservation extenders in different temperatures were selected to compare the semen quality of 8 different extenders in this experiment.The results show that the extender of No.2 and No.8 were the best for diluting semen in the cryopreservation tests,the spermatozoa progressive motility is 0.40 and the percentage of intact acrosome is 49%,and the difference was significant(P0.05).Compared with the control group,the liquid semen could survive longer at the temperature 15℃than any other groups(P0.05),and the extender No.7 was the best for diluting semen in liquid semen preservation tests,the average survival time of sperm was 103 h,there was significant difference compared with control group and other groups(P0.05).