Expression of vascular endothelial growth factor in liver tissue of mice with acute liver injury induced by carbon tetrachloride

Objective To investigate the expression of vascular endothelial growth factor(VEGF) in liver tissue of mice with acute liver injury induced by carbon tetrachloride(CCl4).Methods The mice models of acute liver injury were established by 1 g·L-1 CCl4 induced through the abdominal subcutaneous injection.The enzyme activities of ALT and AST in serum were analyzed and liver pathological changes were also observed by hematoxylin-eosin stain method at 3,6,12,24,30,36,42,48,54 hours after CCl4 injection respectively.The expressions of VEGF protein were determined by Western blot.Results Compared with the negative control group,the enzyme activity of ALT and AST were significantly increased(P0.05) in the model of liver injury at the different time,the highest level at 36 hours after CCl4 induced.The liver tissue structures were all harmed by CCl4 at the different level,especially at 36 hours,reached to the highest level.The expression of VEGF protein was significantly changed and reached the highest level at 24 hours and then gradually decreased to the normal level.Conclusion The expression of VEGF protein is significantly changed and probably plays an important role in the acute liver injury by CCl4.