Effects of variety,culture medium and inoculum source on the growth of virus-free shoots of potato in vitro

Using virus-free shoots in vitro of early maturity potato variety Atlantic, medium maturity variety Kexin 1 and late maturity variety Qingshu 168 as materials,experiments were conducted to study the effects of variety,culture medium and inoculum source on the fresh weight shoot,fresh weight of root,dry weight of seedling,dry weight of root,number of leaves,plant height,stem diameter,root length and number of root,which were investigated at the 14th day. The results showed that,agronomic characters of the three varieties were the best on liquid cultivating medium and the average of per plant fresh weight,dry weight,plant height,stem diameter,node length and root number were: 159.4 mg,9.1 mg,7.3 cm,1.16 mm,1.4 cm and 5.6 bar.The production costs of potato could be reduced under this condition,meanwhile,the cultivating time of virus-free shoots of potato could be shortened by 7～9 days.The inoculum source had significant influence on the per plant root fresh weigh,seedling dry weight,number of leaves,plant height,stem diameter and root length(P0.05),and variety also had significant influence on the fresh weight of seedling,fresh weight of root,dry weight of root,number of leaves,plant height,stem diameter,root length and root number of different maturity varieties(P0.05).Variety and inoculum source had interactive effect on the fresh weight of root,dry weight of seedling,dry weight of root,number of leaves,plant height,stem diameter and root length(P0.05).So they had the best comprehensive agronomic characters when the inoculum density was 0.53/cm2 for early maturity variety Atlantic,and 0.80/cm2 for both medium maturity variety Kexin 1 and late maturity variety Qingshu 168.