Study on HPLC-Fingerprint of Polygonum chinense L.

Objective:To establish the HPLC fingerprint analysis methods for Polygonum chinense L and to provide the reference for quality evaluation of Polygonum chinense L.Method:Chromatographic separation was performed on a Platisil ODS C18 column(4.6 mm×250 mn,5 μm) with acetonitrile-0.05% phosphoric acid as a mobile phase in gradient elution for 60min.The flow rate was 1.0 mL·min-1,detected at wavelength of 360 nm.Result:Seventeen chromatographic peaks were determined for characteristic peak constituted a HPLC fingerprint of P.chinense Similarity evaluation results showed that all P.chinense were above 0.90 in similarity.Conclusion:The method is accurate and reliable;the fingerprints can be used for P.chinense quality control.