The application value of combined detection for serum alpha-fetoprotein,alpha-fetoprotein-L3,Golgi protein 73 and AFP-mRNA in the diagnosis of hepatocellular carcinoma

Objective To investigate the application value of combined detection for alpha fetoprotein (AFP),alpha-fetoprotein-L3 (AFP-L3),Golgi protein 73 (GP73) and AFP-mRNA in the diagnosis of hepatocellular carcinoma (HCC).Methods Enzyme-linked immunosorbent assay (ELISA) was used to detect the serum levels of GP73 in 73 cases of HCC,41 cases of liver cirrhosis,36 cases of chronic hepatitis B and 25 healthy subjects.AFP was detected by chemiluminescent immunoassay,and AFP-L3 was determined by adsorption centrifuging.AFP-mRNA was tested by fluorescent quantitative RT-PCR.Results There were significant differences in the levels of AFP,AFP-L3,GP73,AFP-mRNA between HCC group and cirrhosis group,normal control group (P0.01).The areas evaluated according to ROC curves were 0.691 (AFP),0.859 (APF-L3),0.816 (GP73),0.762 (AFP-mRNA) respectively in HCC group,and the sensitivities were 54.79%,76.71%,80.82%,53.42%,respectively,and specificities were 79.41%,95.10%,82.35%,99.02%,respectively.The areas under ROC curves for the four tumor-related markers were 0.958 by combined detection.The sensitivity of combined detection was significantly increased,which reached 95.8%,and its specificity was 95.0% in the diagnosis of HCC.Conclusion The combination detection for AFP,AFP-L3,GP73 and AFP-mRNA can obviously enhance the sensitivity and specificity in the diagnosis of HCC,which is superior to individual detection of AFP,AFP-L3,GP73 or AFP-mRNA.