Determination of Chloramphenicol in Chloramphenicol Tablets by Internal Standard Method of FTIR

Potassium ferricyanide was added into chloramphenicol samples as internal reference and the mixtures were measured by Fourier transform infrared spectrophotometry(FT-IR). The peak of chloramphenicol at 1686.4 cm-1was chosen as quantitative peak and the one of potassium ferricyanide at 2117.2 cm-1was chosen as inside mark quantitative peak. The ratio of two peak's absorbance(y=hi/hs) had good linear relationship with their mass ratio(x=mi/ms). The linear regression equation was y =1.3127x +0. 5705 with the correlation coefficient r = 0.9981. The contents of chloramphenicol in the samples of chloramphenicol tablets was 77.2%, the relatively standard deviation was 1.8%, the recoveries of the method was 96.8%~103.8%. This method was highly specific measure, especially for fast batch testing.