Analysis of Tebuconazole Residue in Banana and Soil

A method for the determination of tebuconazole residues in banana fruit and flesh and in soil was established,with which the residue dynamics of tebuconazole and its final residue in banana and soil were determined.The samples were extracted with acetonitrile and purified with a Carbon/NH2 SPE column,and then tebuconazole in them was determined by HPLC(high performance liquid chromatography).Two field experiments were conducted in Yunnan and Hainan.The average recoveries of tebuconazole in banana fruit and flesh and soil were found in the range of 80%~107% at the three spray levels from 0.025to0.2mg/kg,the relative standard deviation being 0.7%~3.3%,the lowest detection limit of instrument for tebuconazole being 2×10-10 g and the limit of quantitation being 0.025mg/kg.The field experiment results demonstrated that the half-life of tebuconazole in banana was 16.3din Yunnan and 16.9din Hainan,and its half-life in soil was 16.3din Yunnan and 19.1din Hainan.The residue of tebuconazole was0.05mg/kg in banana harvested 60 and 75dafter pesticide spray.The method showed high accuracy,high sensitivity and good linearity in this study,and is recommended for the work of evaluating the safety and rationality of tebuconazole application in banana production.