2014•Unpublished venueRequires access
Protective effect of reduced glutathione on endotoxin induced myocardial injury in rats
LI Shao-we
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Abstract
Objective:To observe the myocardial injury in rats with endotoxemia, and the intervention effect of GSH and its mechanisms of protection on septic myocardial injury. Methods : Twenty-four Wistar rats were randomly divided into three groups, and eight in each: LPS group:intraperitoneal injection of LPS; LPS + GSH group : intraperitoneal injection of LPS and; normal control group(NS group): intraperitoneal injection of the same dose of NS. Take part of heart HE dyeing to observe the change of myocardial use microscope. Detect the serum cTnI and ET-1 by ELISA; Results: ①LPS group has severe myocardial injury observed under light microscope, LPS + GSH group myocardial tissue injury more severe than Normal control group, but lighter than LPS group. ② Compared with normal control group, serum levels of cTnI and ET-1in the LPS group were significantly higher(P0.01), serum of cTnI and ET-1 in LPS + GSH group were higher than the normal control group,but lower than the LPS group(P0.05). Conclusion: Endothelin myocardial vasoconstriction involved in the pathogenesis of myocardial injury in endotoxemia. GSH can relieve the myocardial injury in rats with endotoxemia, probably through reducing the ET-1 on the expression.