Microwave Extraction and Antioxidant Activity of Total Flavonoid in Blueberry Leaves

In this paper,conditions of microwave extraction of total flavonoid in blueberry leaves were optimized,and its antioxidant activity was evaluated.The optimized conditions obtained using orthogonal design were: microwave power 500 W,ethonal concentration 70 %,extration time 50 s,and the ratio of material to solvent 1∶60(g/mL).Under the conditions,the content of total flavonoid was 30.187 mg/g,significantly higher than that obtained by water extract method,13.415 mg/g.DPPH scavenging test and β-carotene/linoleic acid assay were used to evaluated the antioxidant activity.Compared with BHT and Asc A standards,the extration of blueberry leaves had better antioxidant activity.