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2015•China Foreign Medical TreatmentRequires access

Comparison of Detection of Influenza Viruses between real-time Quantitative Reverse Transcriotion-polymerase Chain Reaction(RT-PCR) and Cell Culture

Jiang Hong-ta

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Abstract

Objective Comparison the difference detection of influenza viruses between real-time quantitative reverse transcriotion-polymerase chain reaction(RT-PCR) and cell culture.Methods Using real-time quantitative RT-PCR and classic MDCK cells virus isolation two ways,censorship influenza surveillance point 80 copies of suspected flu specimens of influenza virus simultaneously.Results Consequence The positive of virus isolation cells is 26 copies.The positive of real-time quantitative RTPCR is 36 copies,x~2=8.1,P0.005.Conclusion Through this experiment,verifying the real-time quantitative RT-PCR is really fast sensitive and suitable for rapid diagnostic laboratory.

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What this paper is about

Objective Comparison the difference detection of influenza viruses between real-time quantitative reverse transcriotion-polymerase chain reaction(RT-PCR) and cell culture.Methods Using real-time quantitative RT-PCR and classic MDCK cells virus isolation two ways,censorship influenza surveillance point 80 copies of suspected flu specimens of influenza virus simultaneously.Results Consequence The positive of virus isolation cells is 26 copies.The positive of real-time quantitative RTPCR is 36 copies,x~2=8.1,P0.005.Conclusion Through this experiment,verifying the real-time quantitative RT-PCR is really fast sensitive and suitable for rapid diagnostic laboratory.

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Available abstract

Objective Comparison the difference detection of influenza viruses between real-time quantitative reverse transcriotion-polymerase chain reaction(RT-PCR) and cell culture.Methods Using real-time quantitative RT-PCR and classic MDCK cells virus isolation two ways,censorship influenza surveillance point 80 copies of suspected flu specimens of influenza virus simultaneously.Results Consequence The positive of virus isolation cells is 26 copies.The positive of real-time quantitative RTPCR is 36 copies,x~2=8.1,P0.005.Conclusion Through this experiment,verifying the real-time quantitative RT-PCR is really fast sensitive and suitable for rapid diagnostic laboratory.

Key concepts: Real-time polymerase chain reaction, Virology, Virus isolation, Polymerase chain reaction, Virus, Isolation (microbiology), Medicine, Reverse transcription polymerase chain reaction

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Comparison of Detection of Influenza Viruses between real-time Quantitative Reverse Transcriotion-polymerase Chain Reaction(RT-PCR) and Cell Culture — Research Paper | ScholarLens