Experimental Study of the Protective Effect of N-Acetylcysteine on Acute Necrotic Pancreatitis

Objective To investigate protective effect and mechanism of N-Acetylcysteine(NAC)on acute necrotic pancreatitis(ANP).Methods Fifty-four SD rats were randomized into three groups:the sham operation(SO)group,ANP group and NAC-pretreated group.The model of ANP was induced by retrograde injection of 5% sodium taurocholate into the bili-pancreatic duct in SD rats,NAC-pretreated group was given 200mg/kg NAC intraperitoneally 30 min before inducing ANP.The levels of amylase,TNF-α,IL-6,malondialdehyde(MDA)in plasm,and myeloperoxidase(MPO)in the lung,the wet /dry ratio of lung were measured.The histopathological changes of pancreatic and pulmonary tissue were evaluated and intrapulmonary TNF-αmRNA was detected.Results The levels of amylase,TNF-α,IL-6,MDA in plasm,the MPO in the lung and wet /dry ratio of lung were significantly increased in ANP group compared with those in the SO group(P0.05),the expression of TNF-αmRNA in the lung was increased significantly in ANP group(P0.01)Compared with the ANP group,the indexes were significantly decreased in the NAC-pretreated group(P0.05),the severity of pancreas and lung injury also significantly decreased in the NAC-pretreated group.Conclusion NAC may have protective effect on ANP by cleaning oxygen free radicals and decreasing the production of TNF-α and IL-6.