2002•PubMedRequires access

[Culture and pluripotentiality of human marrow mesenchymal stem cells].

Wenyu Fu, Yanmeng Lu, Ying-jie Piao

Abstract

OBJECTIVE: To cultivate human mesenchymal stem cells (MSC) derived from fetal bone marrow and examine their pluripotentiality. METHODS: Bone marrow mononuclear cells from human fetal femur were collected by Percoll gradient centrifugation. The low density cells including MSCs were cultivated and expanded in MSCGM media. The characteristics of the multipotent MSCs were observed by implanting them into nude mice for 4 weeks. RESULTS: Human fetal marrow MSCs were successfully cultivated and differentiated in vivo into many kinds of tissues such as bone, cartilage, adipose, skeletal muscle and tendon-like tissue. CONCLUSION: Human fetal marrow MSCs were multipotent stem cells.

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Key concepts: Mesenchymal stem cell, Stem cell transplantation for articular cartilage repair, Bone marrow, Percoll, Multipotent Stem Cell, Amniotic stem cells, Stem cell, Clinical uses of mesenchymal stem cells