Study on the Factors Influencing Abortive Ovules Rescue of "Hanfu" Apple

In this experiment,ovaries and abortive ovules of Hanfu apple were used to investigate the effect of different inoculation time,different basic media,different hormone concentrations and amino acid species,different medium phases,and light/dark condition on development rate of abortive ovules and ovaries culture in vitro.The results indicated that the most suitable medium for ovules growth was Nitsch + folic acid 5 mg/L + IAA 0.5 mg/L + 6-BA 1 mg/L + GA3 2 mg/L at big bud stage and ovary diameter was 0.3 cm(5 days after flower),while the most suitable medium for ovules growth was Nitsch + folic acid 5 mg/L + IAA 1 mg/L + 6-BA 1 mg/L + GA3 2 mg/L at 10 days after flower.The rescue rate with this formula was the highest,reached to 80%.Moreover,in the late stage of abortive ovules development,cysteine and glutamate played a major role in ovules rescue,the training effect of various amino acids was better than single amino acid.Solid-liquid double phase medium had the most positive effect on abortive ovules development.The development rate of abortive ovules under dark condition was higher than under light and dark alternative culture condition.The viability of ovules,which were cultured under dark condition and detected by TTC,was significantly higher than that under light and dark alternative culture condition.