Study on Extraction and Determination of γ-Aminobutyric Acid in Tea

The method has been studied to extractisolate and determine the γ-aminobutyric acid in tea. Taking water or alcohol as solvent, it was discovered that water can achieve a better effect with, γ-aminobutyric acid content to be enhanced 20 %. With the ion exchange resin for isolation and 732 positive ions resin columns (2.5×50 cm) for adsorption, and citric acid and ammonia water as buffer liquids, so as to carry on pH linear gradient elution the results indicated that the γ-aminobutyric acid concentration can reach roughly 39 %. By thin layer scanner to continue the mensuration, the assaying conditions and parameters can be determined.