The Preparation of Lactic Acid Bacteria Powder Starter

In order to get lactic acid bacteria starter with a large number of live cells, the medium and culture conditions were studied in this experiment. Results indicate that the composition of the medium for Lactobacillus fermentum and Lactobacillus plantrum is 2% sucrose, 0.58% peptone and 10.92% soybean powder. A large number of lactic acid bacteria cells can be gained in shorter time with 0.5% KH_2PO_4-Na_2HPO_4 added. The selected medium inoculated with 3% Lactobacillus fermentum and Lactobacillus plantrum are cultivated at 38 ℃ for 18 h, and the cell concentrations can reach 6.02×10~9 and 5.88×10~9 per milliliter medium respectively. Such a method can be used in large-scale production of lactic acid bacteria starter.