2012•China Medical HeraldRequires access
Effect of sodium butyrate on apoptosis of pancreatic cells in rats with severe acute pancreatitis
Huanfeng Tang
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Abstract
Objective To investigate the effects of sodium butyrate on apoptosis of pancreatic cells in rats with severe acute pancreatitis(SAP).Methods 30 SD rats were randomly divided into the control group,SAP model group and NaB treatment group,with 10 cases in each group.The SAP models rats were established by retrograde injection of sodium taurocholate into biliopancreatic duct respectively,sodium butyrate was given to the rats in SAP model group by femoral vein injection 5 minutes after the establishment of SAP model.The levels of serum amylase and the wet/dry weight ratio of pancreas were detected.Pancreatic histopathological scores including edema,hemorrhage,necrosis,inflammatory were evaluated by Kusske method.Apoptosis of pancreatic cells was detected by TUNEL method.Results The serum amylase in SAP group was higher than that in control group,the difference was statistically significant(P 0.01),the serum amylase in NaB treatment group was lower than that in SAP group,the difference was statistically significant(P 0.05);the wet/dry weight ratio in SAP group was higher than that in control group,the difference was statistically significant(P 0.01),the wet/dry weight ratio in NaB treatment group was lower than that in SAP group,the difference was statistically significant(P 0.05);pancreatic injury integral in SAP group was higher than that in control group,the difference was statistically significant(P 0.01),pancreatic injury integral in NaB treatment group was lower than that in SAP group,the difference was statistically significant(P 0.05);the apoptotic index in SAP group was higher than that in control group,the difference was statistically significant(P 0.01),the apoptotic index in NaB treatment group was lower than that in SAP group,the difference was statistically significant(P 0.05).Conclusion NaB can contribute to the apoptosis of rat pancreas acinar cells,alleviate the activation of neutrophil and relieve the severity of SAP.