Esculin,an extract from Fraxinus sielboldiana blume,protects SH-SY5Y cells from MPP~+-induced cytotoxicity

Ojbective:To investigate the effect of Esculin extracted from Fraxinus sielboldiana blume belonging to the Oleaceae family on MPP+-induced cytotoxicity in human neuroblastoma SH-SY5Y cells.Methods: Cell viability was tested when treated with 200 μmol/L concentrations of MPP+ for 48 h by MTT assay.Early apoptosis,late apoptosis/necrosis were analyzed by flow cytometry using Annexin V-FITC and propidium iodide(PI) staining,respectively.Generation of reactive oxygen species(ROS) was assessed by DCFH-DA,an oxidation-sensitive fluorescent probe.To evaluate mitochondrial membrane potential(ΔΨm) by using flow cytometry with the fluorescent dye Rhodamine 123.Results:The MPP+-induced loss of cell viability was significantly increased by Esculin treatment at the concentration of 10-7 mol/L,10-6 mol/L,10-5 and 10-4mol/L.The protective effects of Esculin on MPP+-induced cytotoxicity might be ascribed to its anti-oxidative properties by reducing ROS level and anti-apoptotic effect on protection of ΔΨm.Conclusions:Esculin may provide a useful therapeutic strategy for the treatment of progressive neurodegenerative diseases such as Parkinson′s disease(PD).