Research on the tissue specificity of isozymes of the sea bass(lateolabrax japonicus)

Polyarclamide gel electrophoresis was used to detect the expression of isozymes in 8 different organs or tissues of sea bass(Lateolabrax Japonicus),including,heart,liver,eye crystal,retina,muscle,kidney,gill and pectoral fin.Altogether 10 isozymes including LDH,MDH,ME#alpha#-GPDH,ADH,SDH,SOD,G-6-PDH,EST,GDH were analyzed in 3 electrophoresis buffer sytems.The different expression of these 10 isozymes with high variability was observed in the different tissues.Generally,as the expression of degree of 10 isozymes with high variability was observed in the different tissues.Generally,as the expression of degree of 10 isozymes is concemed,tissue of liver held highest level.Heart,muscle,retina held higher level and eye crystal,gill,pectoral fin kidney with lower level.The 4 isozymes,LDH<MDH,ME and SOD< could be completely detected in 8 tissues.But the degree of expression was quite different.The main loci detected in all the experiments were observed in the tissues of liver and muscle.In addition,the composition of all loci of 10 isozymes,number of alleles per locus and genetype of each locus are also analyzed.