2006•Zhongguo kangfu yixue zazhiRequires access
Effects of combination of Du meridian electroacupuncture and neural stem cells transplanted on the content of nerotrophine-3 and expression of nerotrophine-3 receptor in injured tissue of rat spinal cord transected completely
Zhang Jian Wei
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Abstract
Objective:To explore the effects of combination of Du meridian electroacupuncture and neural stem cells (NSCs) transplanted on the content of nerotrophine-3(NT-3) and expression of nerotrophine-3 receptor(tyrosine protein kinase C,TrkC) in injured tissue of rat spinal cord transected completely. Method:Thirty adult rats were divided into six groups: control 14d group,DU meridian electroacupuncture plus 14d group(EA 14d group),NSCs transplanted 14d group(NSCs 14d group),DU meridian electroacupuncture plus NSCs transplanted 14d group(EA+NSCs transplanted 14d group),NSCs transplated 30d group(NSCs 30d group) and DU meridian electroacupuncture plus NSCs transplanted 30d group (EA+NSCs transplanted 30d group). T10 spinal cord segment of all animals were completely transected. The rats of EA 14d group,EA+NSCs transplanted 14d group and EA+NSCs transplanted 30d group were treated with DU electroacupuncture 5 days after operation. Result:①The content of NT-3 at injured spinal cord tissue was highest in EA+NSCs transplated 14d group, secondly at EA 14d group and NSCs 14d group. The content of NT-3 was lowest in control 14d group.②Transplanted neural stem cells at completely transected site of spinal cord expressed TrkC in NSCs 30d group and EA+NSCs transplanted 30d group. Conclusion:Combination of Du meridian electroacupuncture and neural stem cells transplanted may significantly enhance NT-3 content of the tissue neighboring completely transected site of rat spinal cord. Some transplanted neural stem cells express TrkC in injured site and near tissue of rat spinal cord.