Changes and Significance of Matrix Metalloproteinase and Its Tissue Inhibitor in Plasma and Cardiac Muscle of Rats with Chronic Cardiac Failure Induced by Volume Overload

Objective To observe the changes of matrix metalloproteinase-8(MMP-8) and its tissue inhibitors of metallopreteinase-1(TIMP-1) in plasma and cardiac muscle of rats with chronic cardiac failure induced by volume overload,and to explore those roles in phy-siology of chronic cardiac failure.Methods Seventeen male SD rats were randomly divided into 2 groups as follows:9 shunt rat models were established by abdominal aorta and inferior vena cava shunt operation and 8 rats after sham operation served as controls.Hemodynamic and echocardiographic measurements were obtained 8 weeks after surgery.Meanwhile,MMP-8 and TIMP-1 concentrations in plasma were eva-luated.MMP-8 mRNA and TIMP-1 mRNA expressions were detected by using real time reverse transcription polymerase chain reaction method in cardiac muscle.Results Left ventricular systolic pressure,difference of left ventricular pressure,left ventricular diastolic,left ventricular peak rate of contraction,left ventricular peak rate of relaxation were significantly reduced in the shunt group compared with those in control group(Pa0.05,0.01).Compared with control group,left ventricular end diastolic pressure were significantly increased in shunt group(P0.05).Compared with control group,the plasma levels of MMP-8 and TIMP-1 mRNA increased significantly in shunt group(Pa0.05).The expressions of MMP-8 mRNA and TIMP-1 mRNA in cardiac muscle were similar between the 2 groups(Pa0.05).The ratio of MMP-8/TIMP-1 significantly decreased in shunt group as compared with that in control group,which was more significant in right ventricular than that in left ventricular.Conclusion MMP-8 and TIMP-1 are involved in ventricular remodeling in rats with chronic cardiac failure resulting from volume overload.