Effects of Genistein on Deposition of Beta-amyloid Protein in Blood Capillaries of Ovariectomized Rat Brain

Objective To investigate and compare the effects of 17 beta-estradiol or genistein on the deposition of Aβ in the blood capillaries of the estrogen-deficient rat brain.Methods Long-term endogenous-estrogen-deficiency models of SD rat were established by bilateral ovariectomy.Fiftly healthy SD rats were randomly divided into 5 groups:normal control group(Baseline),sham-operated group(Sham),ovariectomized group(Ovx),Ovx with genistein intervention group(Gen)and Ovx with 17beta-estradiol intervention group(Est).The rats of the 5 groups were sacrified at the 15th week after operation.The expressions of Aβ were showed by the application of the immunohistochemical technique,and investigated by the cell counting and imaging system analysis.Results The numbers and gray scale of the deposition of Aβ-positive cells in the blood capillaries of the hippocampuses in the Ovx group were higher than those in the other four groups,i.e.Baseline,Sham,Gen and Est groups(P0.01).But the numbers and gray scale of Est group,Gen group,Sham group and Baseline group showed no statistical difference(P0.05).Conclusions A long-term deficiency of the endogenous estrogen can lead to a significant increase of Aβ deposition in brain blood capillary,whereas both the 17beta-estradiol intervention and the genistein intervention can prevent it,and the effects of the two interventions are similar.