The biological effect of Pistacia chinensis Bunge by ~(60)Co γ-ray irradiation

Pistacia chinensis Bung seeds were irradiated to 100,200,400,600 and 800 Gy by 60Co γ-rays to study the biological effects.The results show that germination rate and survival rate of Pistacia chinensis seed decreased with increasing doses.The dose 200 Gy group has the highest variation rate and germination rate.The height of M1 seedling decreased with increasing doses.Compared with the control,dwarfing,plant type asymmetry,vein chlorosis,leaf curl,malformation and other phenotypic variation appeared with the irradiation groups of 100-400 kGy.Using RAPD,the M1 plants were investigated.Remarkable variations in the experimental plants were detected by RAPD PCR,but no variation was detected in the control plants.And the 200 kGy group has high frequencies of RAPD variation than the 100 kGy group.