Construction of replication-deficient human IL-10 recombinant adenovirus

Objective To construct recombinant adenovirus containing human IL-10 gene and check IL-10 expression in vitro. Methods The full-length human IL-10 cDNA was removed from pcDNA3IL-10 and linked to Gateway vector. Then the Gateway vector were mixed with the adenovirus desstination vector. The adenorvirus vector containing IL-10 cDNA was produced. Then the recombinant virus DNA was linearized by RE PacI and transfected into the 293A cell line. The replication-incompetent adenovirus containing IL-10 was harvested from the supernatant of 293A cell line. The Bxpc-3 and AR-42J cell lines were infected by the IL-10 adenovirus recombinant virus DNA and the IL-10 protein was extracted and examined with Western blot. Results The recombinant IL-10 replication-incompetent adenovirus was expressed successfully and the titer of the recombinant adenovirus was 2 × 109 PFU/mL. Conclusion The human IL-10 recombinant adenovirus can be used to infect the target cell in vitro.