A study of Y-chromosome microdeletions in the male infertility

Objective: To study associations with azoospermia and oligozoospermia and Y chromosome microdeletions.To establish a method for molecular diagnosis of Y chromosome microdeletions.Methods: Multiplex PCR amplification of 6 STS in AZF regions of the Y chromosome was examined among 53 examples normal karyotype male patients with azoospermia and oligozoospermia and 5 examples normal men.Results: No Y chromosome microdeletions were found among 5 normal men.6 cases exist microdeletions in AZF region among 53 male patients.The microdeletions rate is 11.3%.Conclusion: Microdeletions of Y chromosome is one of the major causes of severe dyszoospermia;the gene of AZF may be plays an important role in the course of spermatogentics.