Molecular Cloning and expression analysis of 3-hydroxy-3-methylglutaryl-CoA Reductase Gene from Eucalyptus urophylla×E.grandis

The gene encoding 3-hydroxy-3-methylglutaryl-CoA reductase(HMGR) was cloned by Rapid Amplification of cDNA Ends(RACE) method,the full-length EuHMGR cDNA was 1955 bp,containing a 1 560 bp open reading frame,encoding a peptide of 519 amino acids.Bioinformatics analysis indicated EuHMGR contained two HMG-CoA binding motifs and two NADP(H) binding motifs;and it had a 3-D structure withVhomology-based on modeling,containing N-domain,S-domain and L-domain.There was a 319 bp intron in EuHMGR gene genomic DNA sequence compared with the cDNA sequence.Tissue expression profile analysis by Real-Time quantitative PCR indicated EuHMGR expression was the highest in stem,followed by leaf and no expression in root.The paper would supply some information to the function analysis and genetic transformation of EuHMGR from Eucalyptus urophylla×E.grandis.